In the world of fitness and nutrition, timing is often emphasized, especially when it comes to protein intake around workouts. Many believe that the timing of protein consumption—whether before or after exercise—can significantly impact muscle growth and performance. However, a new study led by Mohammadyasin Lak and colleagues has shed light on whether timing truly plays a role in maximizing the benefits of a high-protein diet when combined with resistance training
Study Overview
The study, published in Frontiers in Nutrition, aimed to compare the effects of two different timing strategies for protein intake on muscle hypertrophy (growth), strength, and overall performance in resistance-trained males. Over an 8-week period, 40 males with resistance training experience, averaging 24 years of age, were divided into two groups. Both groups consumed 2 grams of protein per kilogram of body weight daily—a high-protein diet known to support muscle growth.
The key difference between the groups was when they consumed their protein:
- Immediate Group: This group consumed protein immediately before and after their workouts.
- 3-Hour Group: This group consumed protein three hours before and after their exercise sessions.
The researchers assessed body composition, muscular performance, and biochemical markers (such as urea levels) before and after the 8-week intervention.
Results
While nine participants withdrew from the study, the remaining 31 completed the full intervention, providing valuable insights into the effects of protein timing.
Across both groups, the study found significant improvements in muscle strength, skeletal muscle mass, and performance in exercises like the Australian pull-up. Interestingly, there were no significant differences between the two groups in terms of muscle growth or performance enhancements, indicating that whether protein was consumed immediately around the workout or a few hours before and after did not make a difference.
Additionally, biochemical markers, particularly urea levels, increased significantly in both groups. This is a normal response to high protein intake, as the body metabolizes and breaks down the amino acids.

Conclusion: Total Protein Intake Matters Most
The results of this study suggest that the timing of protein intake, whether immediately around the workout or a few hours apart, does not significantly impact muscle hypertrophy or performance in resistance-trained males. Instead, the total daily protein intake seems to be the most crucial factor in promoting muscle growth and enhancing strength during resistance training.
For athletes and individuals focused on muscle gain, the key takeaway is clear: prioritizing overall protein consumption throughout the day is more important than worrying about precisely when to consume it in relation to your workouts.
Practical Implications
For those aiming to build muscle or improve performance, this study offers practical and straightforward advice:
- Focus on consuming adequate protein throughout the day to support muscle recovery and growth.
- While timing might have some marginal effects, it is not as crucial as previously thought for trained individuals. Ensuring you meet your daily protein target (2 grams per kilogram of body weight in this study) is the most effective strategy for muscle hypertrophy.
This research adds to the growing body of evidence that debunks the myth of strict “anabolic windows” for protein consumption, offering more flexibility for those trying to maximize their training outcomes.